Major recourses housed at USC Keck Genomics Platform (KGP)
General Laboratory
 Refrigeration |
Refrigeration – Laboratory grade 30cu.ft refrigerators, 2 x 30 cu.ft. freezers and 1 Eppendorf Inova U725 Ultra Low Temperature Freezer that all meet NFPA and OSHA guidelines. |
 Centrifugation |
Centrifugation – large Eppendorf 5810R refrigerated centrifuge, 2 desktop Eppenorf 5424R refrigerated centrifuges, 4 mini MyFuges centrifuges. |
 ThermoMixer |
Thermo blocks – 2 Eppendorf ThermoMixer C 96-well with heated lid Eppendorf ThermoTop. |
Sample Isolation and Preparation
 Bullet Blander |
Bullet Blander – Next Advance capable to homogenize, disrupt, or lyse up to 24 tissue or cell culture samples at a time. |
 PCR |
PCR Farm – comprised of 30 SimpliAmp™ Thermal Cycler (Fisher Scientific, Applied Biosystem® instruments) with 96-well cyclinVeriFlex™ blocks that provide 3 independent temperature zones. |
 Covaris |
Covaris E220 – focused-ultrasonicator (500KHz) capable of processing 96 samples in a single batch. |
Automation – Liquid Handling
 Bravo |
Bravo – Complete Agilent liquid handling solution equipped with high-accuracy pipette heads for 96-well microplates capable of processing 8 x 96 samples that is integrated with PlateLoc Thermal Microplate Sealer with VWorks automation control software for driving the integration. |
Quality Control
 TapeStation |
TapeStation – Agilent 4200 TapeStation system standard for quality control within any Next Generation Sequencing workflows. |
 Fluorometer |
Spectrophotometer/Fluorometer – DeNovix Inc. DS-11 FX+ Spectrophotometer / Fluorometer integrates fluorescence analysis and UV-Vis in the same unit that is coupled with DNA and RNA Fluorescence Assays. |
 qPCR |
Real-Time PCR – QuantStudio 5 Real-Time PCR System, Thermo Fisher Scientific, with 96-well thermal blocks with either 0.1 or 0.2 mL well volume. |
Sequencers
 Illumina NovaSeq 6000 |
NovaSeq 6000 – Illumina NovaSeq 6000 is the newest two color high-throughput sequencer capable of generating up to 6Tb of data with total of 20 billion reads on a single 2-flowcell run in 48 hours. |
 Illumina MiSeq |
MiSeq – Illumina MiSeq benchtop sequencer is optimized for speed and ease of use. It utilizes a single flowcell with single lane with maximum capacity of 25M reads and 15Gb of data per flowcell and up to 39 hours of run time on a 300 pair-end flowcell. It utilizes MiSeq Reporter as the analysis tool. |
Computational Resources
 High Performance Computing |
High Performance Computing – Processing: 720 2.4GHz Intel processors each with 64GB of memory and 2TB internal drive mounted on 36 nodes housed in USC’s HPC cluster and accessed via a private departmental queue. Scheduling: Private departmental queue. Storage: 7 x Dell PowerVault MD1200 with 2 x management modules, 1 x 6Gb 1m SAS cable, mounting rails, two power supplies, 12 x 10TB 7.2K NLSAS 12Gbps 3.5 drives. |
 Servers |
Servers – Processing: 4 x Dell PowerEdge T630 Server Dual Node 40 processor, Intel Xeon E5-2600 v3 2.6Ghz,25M Cache,9.60GT/s QPI,Turbo,HT,10C/20T (105W) Max Mem 2133MHz, 32GB RDIMM,2400MT/S, Dual Rank, x4 Data Width, Storage: 24TB SSD SATA Write Intensive 6Gbps storage. |
Additional Resources
Our colleagues at USC Genome Core UPC Facility Click Here
Our colleagues at USC Molecular Genomics Core Click Here
